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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
10:48

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples

Published on: March 28, 2015

Viremia in hospitalized children with enterovirus infections

R Dagan, J A Jenista, S L Prather

    The Journal of Pediatrics
    |March 1, 1985
    PubMed

    Insights

    Diagnosing enterovirus infections in children is more accurate with blood cultures. This method aids in early presumptive diagnosis, especially in infants presenting with sepsis-like symptoms.

    Area of Science:

    • Pediatrics
    • Infectious Diseases
    • Virology

    Background:

    • Enterovirus infections are common in hospitalized children.
    • Accurate and timely diagnosis is crucial for effective management.
    • Traditional diagnostic methods may not always detect the virus.

    Purpose of the Study:

    • To evaluate the diagnostic utility of blood cultures for enterovirus detection in children.
    • To determine the clinical characteristics associated with enterovirus viremia.
    • To assess the impact of blood culture on the speed and accuracy of diagnosis.

    Main Methods:

    • Prospective study conducted during summer and fall 1982.
    • Collected samples included cerebrospinal fluid (CSF), blood, stool, and throat swabs.
    • Enterovirus isolation and presumptive diagnoses were recorded.

    Main Results:

    • Enterovirus was isolated from 48 hospitalized children; 60% from CSF or blood, 40% with presumptive diagnosis only.
    • Blood cultures were positive in 44% of cases, being the sole positive specimen in two children.
    • Viremia was more frequent in infants <3 months with sepsis-like symptoms and inversely related to CSF pleocytosis.

    Conclusions:

    • Blood culture significantly improves the accuracy of enterovirus disease diagnosis.
    • Adding blood culture to CSF, stool, and throat cultures enhances diagnostic yield.
    • Early diagnosis is achievable, particularly in young infants with suspected bacterial sepsis.

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