A Single-Step Self-Assembly Approach for the Fabrication of Aligned and Multilayered Three-Dimensional Tissue
Yinshen Wee1, Amanda N Moore2, Shihai Jia1
11 School of Dentistry, University of Utah, Salt Lake City, UT, USA.
SLAS Technology
|May 30, 2018
Summary
This study introduces a simple, one-step method using self-assembling multidomain peptide hydrogels to create layered 3D tissue constructs. This technique enhances cell proliferation and tissue remodeling for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Hydrogels are limited in creating oriented multilayered 3D tissue constructs.
- Existing techniques are often complex, require extra devices, and are not widely accessible.
Purpose of the Study:
- To develop a simple, one-step methodology for fabricating 3D tissue constructs with oriented multilayered architectures.
- To investigate the use of self-assembling multidomain peptide (MDP) hydrogels for cell alignment and tissue remodeling.
Main Methods:
- Fabrication of 3D tissue constructs using self-assembling MDP hydrogels.
- Characterization of dental pulp cell viability, structural features, and molecular properties within the hydrogel scaffolds.
- Manipulation of cell layering by adjusting the weight by volume percentage (w/v%) of MDP.
Main Results:
- Demonstrated successful alignment of cells into multiple layers within MDP hydrogels.
- Observed enhanced gene expression related to cell proliferation, angiogenesis, and extracellular matrix (ECM) remodeling.
- Successfully fabricated stratified multilayered and tubular tissue structures.
Conclusions:
- The developed one-step MDP hydrogel methodology offers a simple and rapid approach for generating 3D tissue constructs with controlled multilayered architectures.
- This method effectively mimics in vivo microenvironments, showing potential for modeling complex tissues in regenerative medicine.
Keywords:
3D tissue constructsECM remodelingangiogenesismouse dental pulp cellsself-assembling multidomain peptide hydrogelMore Related Videos
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