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Related Experiment Video

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Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity
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eIF4A2 is a host factor required for efficient HIV-1 replication.

Jerry Kwame Ndzinu1, Hiroaki Takeuchi1, Hideki Saito1

  • 1Department of Molecular Virology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo, 113-8519, Japan.

Microbes and Infection
|May 30, 2018
PubMed
Summary

Human immunodeficiency virus type 1 (HIV-1) replication depends on host factors. This study shows that eukaryotic translation initiation factor 4A isoform 2 (eIF4A2) is essential for efficient HIV-1 replication in T cells.

Keywords:
DEAD-box proteinHIV-1Host factorRNAiViral cDNA synthesiseIF4A2

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Human immunodeficiency virus type 1 (HIV-1) requires host cell factors for replication.
  • Genome-wide RNA interference screening is a method to identify these host factors.

Purpose of the Study:

  • To investigate the role of eukaryotic translation initiation factor 4A isoform 2 (eIF4A2) in HIV-1 replication.
  • To determine if eIF4A2 is a necessary host factor for efficient HIV-1 infection.

Main Methods:

  • Genome-wide RNA interference screening in a human T cell line.
  • Stable depletion of eIF4A2 using lentiviral shRNA in MT4C5 cells.
  • Assessment of HIV-1 replication, viral cDNA synthesis, and infection spread in eIF4A2-depleted cells.

Main Results:

  • Depletion of eIF4A2 significantly inhibited HIV-1 replication.
  • eIF4A2 depletion reduced the efficiency of viral cDNA synthesis.
  • HIV-1 virion entry into target cells was not affected by eIF4A2 depletion.
  • Inhibition of HIV-1 spreading infection was observed in a knockdown level-dependent manner.

Conclusions:

  • HIV-1 requires eIF4A2 for optimal replication in human T cells.
  • eIF4A2 is a critical host factor influencing viral cDNA synthesis during HIV-1 infection.