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A Cyclotide Isolated from Noisettia orchidiflora (Violaceae)
Antonio Fernández Bobey1, Meri Emili Ferreira Pinto1, Eduardo Maffud Cilli1
1NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil.
Planta Medica
|May 30, 2018
Summary
Researchers identified cyclotides, plant defense peptides, in the Brazilian plant *Noisettia orchidiflora*. One cyclotide, Nor A, was isolated and its amino acid sequence determined.
Area of Science:
- Natural Product Chemistry
- Plant Biochemistry
- Medicinal Botany
Background:
- Cyclotides are cyclic peptides found in plants, known for their roles in plant defense.
- Brazilian flora is a rich source of novel bioactive compounds.
- Previous research has explored peptides from various plant species.
Purpose of the Study:
- To investigate cyclotides in the Brazilian plant *Noisettia orchidiflora*.
- To isolate and characterize a specific cyclotide from this species.
- To determine the primary structure of the isolated cyclotide.
Main Methods:
- Liquid Chromatography-Mass Spectrometry (LC-MS) for cyclotide detection.
- Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) for structural analysis.
- Enzymatic digestion (endoGlu-C, trypsin, chymotrypsin) and Edman degradation for sequence determination.
- Amino acid analysis.
Main Results:
- Several cyclotides were detected in leaves and stems of *Noisettia orchidiflora*.
- A cyclotide, named Nor A, was successfully isolated from the stems.
- The primary amino acid sequence of Nor A was elucidated using advanced mass spectrometry techniques and enzymatic fragmentation.
Conclusions:
- The study confirms the presence of cyclotides in *Noisettia orchidiflora*, a member of the Violaceae family.
- Nor A represents a novel cyclotide whose structure has been fully characterized.
- This finding contributes to the understanding of cyclotide diversity in Brazilian plants and their potential bioactivity.
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