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Updated: Feb 9, 2026

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

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Study on the methylation status of SPINT2 gene and its expression in cervical carcinoma

Ning Wang, Yanci Che, Fufen Yin

    Cancer Biomarkers : Section a of Disease Markers
    |May 31, 2018
    PubMed
    Abstract

    Insights

    Aberrant methylation of the SPINT2 gene, influenced by HPV infection, is linked to cervical cancer development. This gene

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Cervical cancer poses a significant global health threat to women.
    • SPINT2, an inhibitor of hepatocyte growth factor activator, is often downregulated or silenced in malignant tumors.

    Purpose of the Study:

    • To investigate the promoter methylation status of the SPINT2 gene in cervical carcinoma.
    • To determine the effect of SPINT2 methylation on its gene expression within cervical cancer.

    Main Methods:

    • Analysis of SPINT2 promoter and first exon methylation in HPV-positive/negative cervical cancer cell lines and tissues.
    • Quantification of SPINT2 gene expression using qRT-PCR in cervical cancer and normal samples.

    Main Results:

    • SPINT2 methylation rates were 54% in cervical cancers versus 25% in normal tissues.
    • HPV E6/E7 infection was associated with altered SPINT2 methylation rates in cell lines.
    • Higher SPINT2 methylation was observed in higher-grade cervical cancers (grade 3 vs. grade 2).

    Conclusions:

    • SPINT2 gene expression is regulated by its methylation status.
    • HPV infection alters the methylation status of SPINT2.
    • Aberrant SPINT2 methylation may contribute to cervical cancer development.
    Keywords:
    5-Aza-CdRHPVSPINT2cervical carcinomamethylation

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