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ARL2 overexpression inhibits glioma proliferation and tumorigenicity via down-regulating AXL

Yulin Wang1, Gefei Guan1, Wen Cheng1

  • 1Department of Neurosurgery, The First Hospital of China Medical University, 155 Nanjingbei Street, Heping, Shenyang, Liaoning, 110001, People's Republic of China.

BMC Cancer
|May 31, 2018
PubMed
Abstract

Insights

ARL2 (ADP-ribosylation factor like protein 2) is down-regulated in glioma, inhibiting tumor growth and spread. This finding suggests ARL2 could be a new therapeutic target for glioma patients.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Glioma, a common adult brain tumor, has a poor prognosis.
  • ARL2, an ARF subfamily GTPase, regulates microtubule dynamics and mitochondrial function.
  • ARL2 is a potential prognostic and therapeutic target in various malignant tumors, but its role in glioma is unclear.

Purpose of the Study:

  • To investigate the expression and functional role of ARL2 in glioma.
  • To determine if ARL2 can serve as a prognostic or therapeutic target for glioma.

Main Methods:

  • ARL2 expression analyzed via RT-PCR, immunohistochemistry, and Western blot in glioma samples.
  • Patient outcomes correlated with ARL2 expression using TCGA, CGGA, and Rembrandt datasets.
  • Functional roles assessed through cell proliferation, colony formation, migration, invasion assays, and in vivo xenograft models following ARL2 overexpression.

Main Results:

  • ARL2 expression is downregulated in glioma and inversely associated with poor prognosis.
  • Overexpression of ARL2 suppressed glioma cell proliferation, colony formation, migration, and invasion.
  • Elevated ARL2 inhibited in vivo glioma cell tumorigenicity.
  • ARL2 was found to regulate AXL expression, a key factor in glioma proliferation and tumorigenicity.

Conclusions:

  • ARL2 inhibits glioma cell proliferation, migration, and tumorigenicity by regulating AXL expression.
  • ARL2 presents potential as a novel prognostic and therapeutic target for glioma.
Keywords:
ARL2AXLBrain cancerGliomaTumorgenecity

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