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Effect of cyclic AMP on acidification in the isolated turtle bladder
Kidney International
|January 1, 1985
Summary
Cyclic adenosine monophosphate (cAMP) does not affect hydrogen ion or sodium transport in turtle bladders. However, cAMP stimulates active bicarbonate secretion in bicarbonate-loaded turtles.
Area of Science:
- Physiology
- Renal Physiology
- Ion Transport
Background:
- Cyclic adenosine monophosphate (cAMP) is a crucial second messenger involved in various cellular processes.
- Previous studies suggested cAMP inhibits hydrogen ion secretion in the turtle bladder.
Purpose of the Study:
- To investigate the effect of cAMP on hydrogen ion secretion and sodium transport in the isolated turtle bladder.
- To determine if cAMP influences bicarbonate secretion under specific conditions.
Main Methods:
- Utilized pH stat and reverse short circuit current techniques to measure hydrogen ion secretion and sodium transport.
- Experiments were conducted on isolated turtle bladders under varying CO2 conditions (0% and 1%).
- Administered cAMP and dibutyryl cAMP to serosal or mucosal solutions, with or without theophylline.
Main Results:
- Cyclic AMP (cAMP) and dibutyryl cAMP did not alter hydrogen ion secretion or sodium transport in fasted turtles, irrespective of CO2 levels.
- Theophylline did not affect proton secretion.
- In bicarbonate-loaded turtles, cAMP significantly stimulated active bicarbonate secretion when a 20 mM bicarbonate gradient was present.
Conclusions:
- Cyclic AMP (cAMP) does not influence hydrogen ion secretion or sodium transport in the turtle bladder.
- Cyclic AMP (cAMP) actively stimulates bicarbonate secretion in the turtle bladder, particularly under conditions of bicarbonate loading and a gradient.