Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as

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Qian-Cheng Zhou1, Xue-Feng Deng2, Juan Yang1

  • 1College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, P.R. China.

Oncology Letters
|May 31, 2018
PubMed

Insights

The DEK protein is highly expressed in lung cancer tissues, promoting tumor cell proliferation and invasion. This study investigated DEK

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • DEK protein is ubiquitously expressed and involved in crucial cellular processes like proliferation and DNA repair.
  • Its role in lung cancer carcinogenesis remains understudied despite its association with tumor cells.

Purpose of the Study:

  • To investigate the expression levels of DEK mRNA and protein in lung cancer.
  • To determine the functional role of DEK in lung cancer cell proliferation and invasion.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to measure DEK mRNA levels.
  • Immunohistochemical staining to assess DEK protein expression.
  • In vitro assays (MTT, colony formation, Matrigel invasion) in A549 cells with DEK knock-down and overexpression.

Main Results:

  • DEK expression (mRNA and protein) was significantly increased in lung cancer tissues compared to non-cancerous counterparts.
  • DEK knockdown inhibited, while overexpression enhanced, lung cancer cell proliferation and invasion.
  • DEK positively regulates cell proliferation and invasion in lung cancer.

Conclusions:

  • DEK is upregulated in lung cancer tissues.
  • DEK plays a significant role in promoting lung cancer cell proliferation and invasion.
  • Targeting DEK may offer a potential therapeutic strategy for lung cancer.
Keywords:
DEKcarcinogenesislung cancer

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