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Nickel chloride administration prevents the growth of oral squamous cell carcinoma

Hirotaka Ota1,2, Takashi Shionome3, Hisashi Suguro4,5

  • 1Department of Pathology, Nihon University School of Dentistry, Tokyo, Japan.

Oncotarget
|May 31, 2018
PubMed

Insights

Nickel chloride (NiCl2) significantly inhibited oral cancer progression and metastasis in preclinical models. NiCl2 reduced tumor growth, induced necrosis, and suppressed key genes involved in angiogenesis and cancer spread.

Area of Science:

  • Oncology
  • Biochemistry
  • Molecular Biology

Background:

  • Oral squamous cell carcinoma (OSCC) is a significant global health concern.
  • Metastasis is a primary driver of mortality in OSCC.
  • Novel therapeutic strategies are needed to improve OSCC treatment outcomes.

Purpose of the Study:

  • To investigate the anti-cancer effects of nickel chloride (NiCl2) on OSCC.
  • To evaluate the impact of NiCl2 on tumor growth, angiogenesis, and metastasis in vivo.
  • To explore the potential of NiCl2 as a therapeutic agent for oral cancer.

Main Methods:

  • In vitro studies using the HSC3 OSCC cell line to assess MMP expression.
  • In vivo orthotopic implantation model in nude mice using the HSC3-M3 subline.
  • Treatment with NiCl2-containing water in mice.
  • Immunohistochemistry, CT imaging, real-time PCR, and PCR array analysis to evaluate tumor characteristics and gene expression.

Main Results:

  • NiCl2 significantly reduced matrix metalloproteinase (MMP) expression at both mRNA and protein levels.
  • In vivo, NiCl2 treatment led to reduced tumor mass, increased necrosis, and suppressed expression of angiogenic factors (IL-8, VEGF).
  • NiCl2 administration completely inhibited human beta-globin gene expression in lymph nodes, indicating a blockade of metastasis. Gene expression analysis revealed inhibition of various metastasis-related genes.

Conclusions:

  • NiCl2 demonstrates potent anti-cancer properties against oral squamous cell carcinoma.
  • NiCl2 effectively inhibits tumor growth, angiogenesis, and metastasis.
  • NiCl2 represents a promising therapeutic candidate for oral cancer treatment.
Keywords:
matrix metalloproteinasenickel chloridenuclear factor-kappa Bnude mouseoral squamous cell carcinoma

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