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MicroRNA‑655 inhibits cell proliferation and invasion in epithelial ovarian cancer by directly targeting vascular

Ziling Zhao1, Shuna Yang1, Yan Cheng1

  • 1Department of Obstetrics and Gynecology, Yidu Central Hospital of Weifang, Weifang, Shandong 262550, P.R. China.

Molecular Medicine Reports
|May 31, 2018
PubMed

Insights

MicroRNAs (miRNAs) are deregulated in epithelial ovarian cancer (EOC). Restoring miR-655-3p (miR-655) inhibits EOC cell proliferation and invasion by targeting vascular endothelial growth factor A (VEGF).

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are implicated in epithelial ovarian cancer (EOC) development and progression.
  • miRNA deregulation influences cancer-related gene expression, necessitating further investigation into their roles and mechanisms in EOC.
  • Identifying novel miRNA targets is crucial for EOC diagnosis, prognosis, and therapeutic strategies.

Purpose of the Study:

  • To investigate the role of miR-655-3p (miR-655) in epithelial ovarian cancer (EOC).
  • To identify the direct target genes of miR-655 in EOC cells.
  • To explore the therapeutic potential of the miR-655/VEGF pathway in EOC.

Main Methods:

  • Quantitative analysis of miR-655 expression in EOC tissues and cell lines.
  • Functional assays (proliferation, invasion) following miR-655 restoration.
  • Bioinformatic analysis and experimental validation to identify direct miR-655 targets.
  • VEGF knockdown and overexpression experiments to assess its role in EOC progression.

Main Results:

  • miR-655 expression was significantly downregulated in EOC tissues and cell lines.
  • Restoration of miR-655 suppressed EOC cell proliferation and invasion.
  • Vascular Endothelial Growth Factor A (VEGF) was identified as a direct target of miR-655.
  • VEGF knockdown mimicked the tumor-suppressive effects of miR-655, and VEGF introduction reversed these effects.

Conclusions:

  • miR-655 inhibits EOC cell proliferation and invasion by repressing VEGF expression.
  • The miR-655/VEGF pathway represents a potential novel therapeutic target for epithelial ovarian cancer.
  • This study highlights miR-655 as a tumor suppressor in EOC with diagnostic and therapeutic implications.

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