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Updated: Feb 9, 2026

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
Caspase‑9 was involved in cell apoptosis in human dental pulp stem cells from deciduous teeth
Hong Qian1, Qun Huang2, Yu-Xiang Chen2
1Department of Pediatric Dentistry, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China.
Abstract:
As one type of adult stem cells (ASCs), human dental pulp stem cells (HDPSCs) have several properties, including high proliferation rate, self‑renewal capability, and multi‑lineage differentiation. However, the apoptotic mechanism underlying the development of dental pulp cells remains unclear. In the present study, a significant increase of apoptosis was observed in HDPSCs from the deciduous teeth compared with that from adult permanent teeth. In addition, the occurrence of cytochrome c expression and mitochondrial‑mediated apoptosis pathway activity in HDPSCs were confirmed by quantitative polymerase chain reaction, and western blotting. Although caspase‑8 and caspase‑9 showed higher expression in deciduous teeth than in adult permanent teeth, only the knockdown of caspase‑9 via RNA interference in HDPSC cells exhibited a significant reduction in apoptosis, and caspase‑3 expression and activity. All these results revealed that caspase‑9 and activated caspase‑3 predominantly regulates cell apoptosis in HDPSCs from deciduous teeth.
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