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A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
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Setting Standards for Reporting and Quantification in Fluorescence-Guided Surgery.
Charlotte Hoogstins1,2, Jan Jaap Burggraaf1, Marjory Koller3
1Department of Surgery, Leiden University Medical Center, Albinusdreef 2, 2333, ZA, Leiden, the Netherlands.
Molecular Imaging and Biology
|May 31, 2018
Summary
Standardized methods are needed for intraoperative fluorescence imaging (FI) system assessment. This study introduces a calibration device and analysis techniques to quantify imaging performance and improve signal-to-background ratio (SBR) for better surgical guidance.
Area of Science:
- Medical imaging
- Surgical oncology
Background:
- Intraoperative fluorescence imaging (FI) shows promise for guiding oncologic surgery and improving resection outcomes.
- Current clinical trials and available technologies for FI are increasing, but lack standardized performance assessment and image analysis methods.
Purpose of the Study:
- To systematically compare two commercial FI imaging systems.
- To develop and evaluate a novel calibration device for FI systems.
- To analyze determinants of signal-to-background ratio (SBR) in FI.
Main Methods:
- Conducted a controlled comparison of two commercial FI imaging systems.
- Utilized a novel calibration device for quantifying imaging system performance.
- Analyzed fluorescence images from prior studies to assess SBR and its influencing factors.
Main Results:
- The calibration device enabled quantitative comparison of imaging system sensitivity.
- Image analysis revealed significant impact of background noise and selection on SBR.
- Identified key differences in performance between the evaluated imaging systems.
Conclusions:
- Proposed clear approaches for quantifying FI imaging system performance.
- Suggested standardized methods for post-acquisition image analysis in FI.
- Aimed to establish new standards for intraoperative fluorescence imaging.
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