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Updated: Feb 9, 2026
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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
Published on: April 27, 2018
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A Simple and Sensitive Plant-Based Western Corn Rootworm Bioassay Method for Resistance Determination and Event
1Syngenta Crop Protection, LLC, Davis Drive, Research Triangle Park, NC.
Journal of Economic Entomology
|May 31, 2018
Summary
A new plant-based bioassay effectively evaluates western corn rootworm (Diabrotica virgifera virgifera) in corn. This method assesses insect and plant health, confirming eCry3.1Ab corn efficacy and resistance.
Area of Science:
- Agricultural Entomology
- Plant Biotechnology
- Insecticide Resistance Management
Background:
- Western corn rootworm (Diabrotica virgifera virgifera) poses significant threats to maize cultivation.
- Developing efficient bioassays is crucial for evaluating insecticidal traits in crops and understanding resistance mechanisms.
Purpose of the Study:
- To introduce a novel, sensitive, plant-based bioassay for assessing western corn rootworm.
- To evaluate the efficacy of eCry3.1Ab-expressing corn against this pest.
- To validate the bioassay's capability in determining insecticide resistance.
Main Methods:
- A single experimental setup allowing simultaneous evaluation of plant (root injury, weight, protein expression) and insect (survival, growth, development) parameters.
- Utilized eCry3.1Ab-expressing corn (5307) and a known resistant western corn rootworm strain.
Main Results:
- The bioassay demonstrated high mortality and significantly inhibited larval growth and development in western corn rootworm exposed to eCry3.1Ab corn.
- The method successfully identified resistance in a resistant western corn rootworm strain, validating its utility.
Conclusions:
- The developed plant-based bioassay is a simple, sensitive, and versatile tool for evaluating insecticidal traits in crops.
- This method aids in selecting effective transgenic events and monitoring insecticide resistance in western corn rootworm populations.
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