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Updated: Feb 9, 2026
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
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Multi-Parametric Screening in Arabidopsis thaliana Seedlings
Long Nguyen1, Andrzej Drozdzecki1, Vera Goossens2
1VIB Screening Core, Ghent, Belgium.
Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2018
Summary
We developed a cost-effective, automated method for high-throughput plant phenotyping using Arabidopsis thaliana seedlings. This approach simplifies assessing phenotypic effects and aids in identifying chemical probes for biological pathway research.
Area of Science:
- Plant biology
- Chemical biology
- High-throughput screening
Background:
- Phenotypic screening is crucial for identifying chemical probes to study biological pathways.
- High-throughput assessment of plant phenotypes is challenging and often requires expensive equipment.
Purpose of the Study:
- To describe a cost-effective, multi-parametric screening procedure for automated plant phenotyping.
- To enable high-throughput assessment of phenotypes in Arabidopsis thaliana seedlings.
Main Methods:
- Developed a cost-effective, multi-parametric screening procedure.
- Ensured compatibility with liquid-handling systems for automation.
- Applied the method to assess phenotypes in Arabidopsis thaliana seedlings.
Main Results:
- The procedure allows for automated, high-throughput assessment of plant phenotypes.
- The method is cost-effective compared to traditional readout devices.
- Facilitates the exploration of connections between phenotypes and biological pathways.
Conclusions:
- The described procedure offers a valuable tool for automated, cost-effective plant phenotyping.
- This method supports the identification of chemical probes and the study of plant biological pathways.
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