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Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen
Roman Chuprov-Netochin1, Elena Marusich1, Yaroslav Neskorodov2
1Moscow Institute of Physics and Technology, Moscow Region, Russian Federation.
Abstract:
Chemical genomics attracts considerable attention by offering crucial tools for plant biology to regulate plant growth and development. However, most chemical screens are time consuming and laborious, and require a high input of resources. Here we describe a broadly applicable method for the ultrarapid high-content phenotypic screening of small chemical compound libraries for new plant growth regulators. The assay is based on determination of pollen tube growth and can be completed in less than 8 h. Using this method, we identified novel inhibitors and modulators of plant growth and showed that compounds selected using a Nicotiana tabacum-based assay were biologically active in other plants, such as Arabidopsis thaliana.
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