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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Sequences01:29

Sequences

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Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Arithmetic Sequences01:30

Arithmetic Sequences

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An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
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Surface Tension and Surface Energy01:16

Surface Tension and Surface Energy

3.3K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
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Related Experiment Video

Updated: Feb 9, 2026

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

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Next-generation sequencing library construction on a surface.

Kuan Feng1, Justin Costa2, Jeremy S Edwards3,4,5,6

  • 1Chemistry and Chemical Biology, University of New Mexico, Albuquerque, NM, 87131, USA.

BMC Genomics
|June 1, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for DNA library preparation using surface-bound transposases, streamlining the next-generation sequencing (NGS) workflow. This technique simplifies library construction directly on a flowcell, reducing time, cost, and PCR duplicates.

Keywords:
Next generation sequencingSurface reactionTransposases

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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Next-generation sequencing (NGS) is crucial for analyzing genetic variation across biology, agriculture, and medicine.
  • Current NGS workflows involve time-consuming, hands-on DNA library preparation steps.
  • Existing methods require significant manual effort and can introduce biases.

Purpose of the Study:

  • To develop a streamlined and robust method for DNA library preparation.
  • To simplify the construction of DNA libraries for NGS.
  • To reduce the hands-on time and cost associated with library preparation.

Main Methods:

  • Utilized surface-bound transposases for direct DNA fragmentation and library attachment.
  • Constructed DNA libraries directly on a flowcell surface.
  • Applied a novel surface tagmentation approach for library preparation.

Main Results:

  • Successfully generated and analyzed a Drosophila genome library using the surface tagmentation method.
  • Demonstrated comparable library quality to commercially available kits.
  • Eliminated the need for PCR amplification, thereby avoiding PCR duplicates.

Conclusions:

  • Presented the first method for constructing DNA libraries directly on a flowcell.
  • The technique offers potential for integration into existing Illumina sequencing pipelines.
  • The approach can simplify workflows, reduce costs, and enhance data quality in NGS.