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Updated: Feb 9, 2026

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
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A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Published on: March 27, 2017

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Amyloid-beta Interactions with ABC Transporters and Resistance Modifiers

Joseph Molnar1, Imre Ocsovszki2, Rozalia Pusztai3

  • 1Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary molnar.jozsef@med.u-szeged.hu.

Anticancer Research
|June 1, 2018
PubMed
Abstract

Insights

Amyloid-beta complexes with ABC transporter inhibitors reduced multidrug resistance in tumor cells by inhibiting efflux pumps. This suggests a potential link between reduced membrane transport and lower cancer incidence in Alzheimer's disease.

Area of Science:

  • Biochemistry
  • Oncology
  • Neuroscience

Background:

  • Multidrug resistance (MDR) in cancer is mediated by ATP-binding cassette (ABC) transporters, hindering chemotherapy efficacy.
  • Similar ABC transporters are involved in clearing amyloid deposits in Alzheimer's disease (AD).

Purpose of the Study:

  • To investigate the effects of amyloid-beta complexes with ABC transporter inhibitors on MDR tumor cells.
  • To assess the potential of these complexes in reversing multidrug resistance and inducing apoptosis.

Main Methods:

  • Rhodamine-123 accumulation was used to measure efflux pump activity in treated MDR cells.
  • Annexin-V and propidium iodide staining assessed apoptosis induction.
  • Flow cytometry (PARTEC FACScan) analyzed cellular fluorescence.

Main Results:

  • Disiloxanes and memantine complexed with amyloid-beta 1-42 decreased ABC transporter activity in MDR tumor cells.
  • Amyloid-beta complexes moderately increased early apoptosis.
  • No significant changes were observed in late apoptosis or total viable cell count.

Conclusions:

  • Amyloid-beta and its complexes inactivate tumor cell efflux pumps, leading to amyloid accumulation.
  • Reduced membrane transport activity may contribute to the observed lower cancer incidence in Alzheimer's disease patients.
Keywords:
ABC transporterAlzheimer's diseaseamyloid-beta peptideamyloid-memantine and amyloid-disiloxane complexeshuman MDR1 gene transfected mouse lymphoma cell

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