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Differential Expression of IGF-I Transcripts in Bladder Cancer
Nikos Mourmouras1, Anastassios Philippou1, Panagiotis Christopoulos1
1Department of Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Anticancer Research
|June 1, 2018
Summary
Insulin-like growth factor-I (IGF-I) transcripts show altered expression in bladder cancer. Specifically, IGF-IEc expression is suppressed in advanced bladder cancer, suggesting a role in disease progression.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Differential expression of insulin-like growth factor-I (IGF-I) transcripts (IGF-IEa, IGF-IEb, IGF-IEc) is observed in normal and cancerous tissues.
- These transcripts may have distinct biological roles beyond IGF-I.
Purpose of the Study:
- To investigate the expression profile of IGF-IEa, IGF-IEb, and IGF-IEc transcripts in bladder cancer.
- To compare their expression in cancerous tissues versus normal adjacent bladder tissue.
Main Methods:
- Semi-quantitative real-time PCR (qRT-PCR) was used to analyze IGF-I transcript expression.
- Biopsies from 46 bladder cancer patients and adjacent normal tissues were analyzed.
Main Results:
- All three IGF-I transcripts were detected in both normal and cancerous bladder tissues.
- IGF-IEa and IGF-IEb showed a marginal increase in bladder cancer (p>0.05).
- IGF-IEc expression was significantly decreased in bladder cancer (p<0.05) and correlated with advanced disease characteristics (stage, grade, recurrence).
Conclusions:
- Bladder cancer exhibits differential expression of IGF-I transcripts, notably a suppression of IGF-IEc.
- IGF-IEc and its putative Ec product may play a role in bladder cancer progression beyond the general function of IGF-I.
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