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Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
Published on: May 11, 2018
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Solid surface fluorescence immunosensor for ultrasensitive detection of hepatitis B virus surface antigen using
Bahareh Babamiri1, Rahman Hallaj1,2, Abdollah Salimi1,2
1Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Methods and Applications in Fluorescence
|June 1, 2018
Summary
This study developed a highly sensitive fluorescence immunosensor using CdTe@CdS-PAMAM nanostructures for detecting Hepatitis B surface antigen (HBsAg). The novel nanoprobe offers excellent analytical performance, paving the way for early hepatitis B diagnosis.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Hepatitis B virus (HBV) infection poses a significant global health challenge.
- Early and accurate diagnosis of HBV is crucial for effective treatment and management.
- Current diagnostic methods may require further improvements in sensitivity and speed.
Purpose of the Study:
- To develop an ultrasensitive solid-surface fluorescence-immunosensor for Hepatitis B surface antigen (HBsAg) detection.
- To utilize CdTe@CdS-PAMAM nanostructures as a signal-amplifying nanoprobe.
- To enhance the sensitivity and analytical performance for HBsAg quantification.
Main Methods:
- Construction of a fluorescence-immunosensor using CdTe@CdS quantum dots (QDs) covalently attached to PAMAM dendrimers.
- Application of a sandwich immunoassay format involving HBsAg and a PAMAM-QD-antibody conjugate.
- Monitoring of fluorescence intensity for HBsAg determination.
Main Results:
- The developed immunosensor demonstrated high sensitivity for HBsAg detection.
- An excellent linear range was achieved from 5 fg/mL to 0.15 ng/mL.
- A low limit of detection (LOD) of 0.6 fg/mL was obtained at a signal-to-noise ratio of 3.
Conclusions:
- The CdTe@CdS-PAMAM based fluorescence-immunosensor offers a highly sensitive and reliable method for HBsAg detection.
- This assay shows significant potential for the early diagnosis of hepatitis B.
- The platform can be adapted for detecting other tumor markers in clinical settings.
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