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Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells

Zhuohong Li1, Chaofu Zhu1, Baiping An1

  • 1Department of Oncology, The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Oncotargets and Therapy
|June 1, 2018
PubMed
Abstract

Insights

Indirubin, a compound from traditional Chinese medicine, effectively inhibits the proliferation, migration, invasion, and angiogenesis of tumor-derived endothelial cells (Td-EC). This suggests potential for new hepatocellular carcinoma treatments.

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Hepatocellular carcinoma (HCC) is a prevalent malignancy with increasing incidence and high mortality.
  • HCC exhibits resistance to conventional radio- and chemotherapy.
  • Indirubin, a key component of traditional Chinese herbal medicine, possesses anti-tumor properties.

Purpose of the Study:

  • To investigate the anti-cancer effects of indirubin on tumor-derived endothelial cells (Td-EC).
  • To analyze indirubin's impact on Td-EC proliferation, migration, invasion, and angiogenesis.
  • To compare indirubin's efficacy on Td-EC versus normal human umbilical vein endothelial cells (HUVEC).

Main Methods:

  • Td-EC were generated from HUVEC using conditioned medium from the HepG2 human liver cancer cell line.
  • Cell proliferation was measured using the MTT assay.
  • Migration, invasion, and angiogenesis were assessed via wound healing, in vitro invasion, and in vitro tube formation assays, respectively.

Main Results:

  • Successful derivation of Td-EC was confirmed.
  • Indirubin demonstrated significant dose- and time-dependent inhibition of Td-EC proliferation.
  • Indirubin markedly suppressed Td-EC migration, invasion, and angiogenesis, with stronger effects compared to HUVEC.

Conclusions:

  • Indirubin exhibits potent inhibitory effects on Td-EC proliferation, migration, invasion, and angiogenesis.
  • These findings highlight indirubin's potential as a therapeutic agent against hepatocellular carcinoma by targeting tumor angiogenesis and metastasis.
Keywords:
Td-ECangiogenesisindirubininvasionmigrationproliferation

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