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Updated: Feb 9, 2026

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
Published on: August 12, 2016
Modulation of Glucose Takeup by Glucose Transport on the Isolated OHCs
Xiao-Ting Cheng1,2, Feng-Bo Yang1,3, Qing-Qing Jiang1
1Department of Otorhinolaryngology Head and Neck Surgery and Institute of Otorhinolaryngology, The PLA General Hospital, Beijing 100853, China.
Abstract:
Glucose is a fundamental source of energy for mammalian cells; however, whether glucose is taken up through the lateral walls of cochlear outer hair cells (OHCs) is unknown. The OHC lateral wall is complex, composed of a plasma membrane, cortical lattice, and subsurface cisternae. This study assessed the uptake of glucose by OHCs using live-cell microscopy and examined the distribution of glucose transporter isoforms by immunohistochemistry. We found that glucose transporter-4 was mostly expressed on the lateral wall of OHCs. Glucose crossed the lateral walls of OHCs via glucose transporters-4 mainly, and this process could be modulated. These results suggest that the lateral walls are involved in modulating energy transport into OHCs.
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