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Immunological and biochemical characterization of human adenoviruses from subgenus B. II. DNA restriction analysis
Abstract:
47 adenovirus strains related to Ad11, 14, 16, 21, 34, and 35 (including all subgenus B prototypes) were analyzed by DNA restriction analysis with Bam HI, Bgl II, Hind III, and Sma I. Typical fragments occurring in most of the strains were observed only with Hind III. Besides Ad3 and 7, 20 different genome types were distinguished. A pairwise analysis of comigrating fragments was performed for all genome types and summarized for the four enzymes. A medium rate of comigrating fragments (50 to 60 per cent) was found between Ad11, 14, 34, and 35 strains; Ad16 and 21 had 35 to 50 percent comigrating fragments. Within each group of closely related strains, Ad16 strains, Ad21 strains, and Ad34 + 35 strains showed a high rate (greater than 70 per cent) of comigrating fragments. Ad3 and 7 showed more comigration with Ad16 and 21 than with the others. Sixteen strains related to Ad21 were identical in their DNA restriction pattern and by serology. The relatedness determined by fragment comigration and by serology was compared by a quantitative estimate. The DNA relationship between Ad11 + 14 and Ad34 + 35 was not corroborated by serology. Ad21 was serologically related to Ad34 and 35, but not to Ad16; the opposite was found for DNA. The intermediate Ad21-16 showed a unique DNA pattern with some relation to Ad16.
Insights
DNA restriction analysis of 47 adenovirus strains revealed distinct genome types. While serology and DNA analysis showed some discrepancies, this study clarifies adenovirus B subgenus relationships.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Adenoviruses, particularly subgenus B, pose public health challenges.
- Accurate classification of adenovirus strains is crucial for understanding transmission and developing countermeasures.
Purpose of the Study:
- To classify adenovirus strains within subgenus B using DNA restriction analysis.
- To compare DNA-based relatedness with serological classifications.
Main Methods:
- DNA restriction analysis of 47 adenovirus strains using Bam HI, Bgl II, Hind III, and Sma I enzymes.
- Pairwise comparison of comigrating DNA fragments to determine genome types.
- Quantitative comparison of DNA-based relatedness with serological data.
Main Results:
- Twenty distinct adenovirus genome types were identified among the analyzed strains.
- High DNA fragment comigration rates were observed within closely related strain groups (Ad16, Ad21, Ad34+35).
- Discrepancies were noted between DNA and serological relatedness for certain strains (e.g., Ad11+14 vs. Ad34+35, Ad21 vs. Ad16).
Conclusions:
- DNA restriction analysis provides a robust method for distinguishing adenovirus genome types within subgenus B.
- Serological and DNA-based classifications do not always align, highlighting the need for integrated approaches.
- The study identified unique DNA patterns for certain strains, like the Ad21-16 intermediate.