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Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages

Roberto Rosales-Reyes1, Concepción Sánchez-Gómez2, Vianney Ortiz-Navarrete3

  • 1Laboratorio de Infectología, Microbiología e Inmunología Clínicas, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Biomed Research International
|June 1, 2018
PubMed

Insights

Burkholderia cenocepacia enters macrophages via macropinocytosis, a process involving membrane ruffling and macropinosome formation. The Type 3 Secretion System (T3SS) is crucial for this bacterial entry mechanism.

Area of Science:

  • Microbiology
  • Cell Biology
  • Immunology

Background:

  • Burkholderia cenocepacia is an opportunistic pathogen impacting immunocompromised individuals.
  • B. cenocepacia survives within macrophages by arresting vacuole maturation, but entry mechanisms were unclear.

Purpose of the Study:

  • To elucidate the mechanism of B. cenocepacia entry into macrophages.
  • To investigate the role of the Type 3 Secretion System (T3SS) in macrophage invasion.

Main Methods:

  • Observing macrophage-host interactions upon B. cenocepacia exposure.
  • Analyzing the formation of membrane ruffles and macropinosomes.
  • Assessing the contribution of the T3SS to bacterial internalization.

Main Results:

  • B. cenocepacia induces localized membrane ruffling and macropinocytosis upon contact with macrophages.
  • Macrophage entry occurs through the formation of macropinosome-like compartments.
  • The T3SS of B. cenocepacia is essential for both macrophage entry and macropinosome formation.

Conclusions:

  • B. cenocepacia utilizes macropinocytosis for entry into host macrophages.
  • The T3SS plays a significant role in mediating this invasion pathway.

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