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Updated: Feb 9, 2026
14:29
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Published on: May 1, 2013
14.8K
Chromatin interacting factor OsVIL2 increases biomass and rice grain yield
Jungil Yang1, Lae-Hyeon Cho1, Jinmi Yoon1
1Crop Biotech Institute, Kyung Hee University, Yongin, Korea.
Plant Biotechnology Journal
|June 1, 2018
Summary
Oryza sativa VIN3-LIKE 2 (OsVIL2) gene regulates rice yield. Suppressing OsCKX2 chromatin increases cytokinin levels, enhancing plant biomass and grain number in rice.
Area of Science:
- Plant Biology
- Agronomy
- Molecular Genetics
Background:
- Grain number is a crucial agronomic trait influencing rice yield.
- The chromatin interacting factor OsVIL2 plays a role in regulating plant biomass and yield in rice.
Purpose of the Study:
- To investigate the function of OsVIL2 in rice growth and development.
- To elucidate the molecular mechanism by which OsVIL2 affects biomass production and grain number.
Main Methods:
- Gene mutation and overexpression (OX) in rice.
- RNA-sequencing to analyze gene expression changes in OX plants.
- Chromatin immunoprecipitation (ChIP) to identify direct OsVIL2 targets.
Main Results:
- OsVIL2 mutations reduced plant height and grain number; OX enhanced biomass and grain number.
- RNA-seq revealed significant up- and down-regulation of genes in OX plants.
- ChIP analysis confirmed OsVIL2 directly binds to the promoter of CYTOKININ OXIDASE/DEHYDROGENASE2 (OsCKX2), a cytokinin degradation gene.
- Active cytokinin levels were elevated in OX plants.
Conclusions:
- OsVIL2 enhances rice biomass and grain production by suppressing the chromatin of OsCKX2.
- This mechanism involves the regulation of cytokinin levels, highlighting OsVIL2 as a key factor in improving rice yield potential.
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