Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Chromatographic Resolution01:15

Chromatographic Resolution

2.2K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
2.2K
Racemic Mixtures and the Resolution of Enantiomers02:30

Racemic Mixtures and the Resolution of Enantiomers

21.8K
A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit...
21.8K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.6K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiotemporal coordination of Slit-Robo repulsion and neurturin-Gfrα attraction guides multipolar migration during retinal lamination.

Cell reports·2026
Same author

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.

Nature communications·2025
Same author

Ultrasound-guided PRP for coracoid syndrome: a novel case of conjoint tendon enthesopathy.

Pain management·2025
Same author

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Nature methods·2025
Same author

Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.

Nature protocols·2025
Same author

Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy.

Nature communications·2025
Same journal

Corrigendum to "Sodium butyrate down-regulation of indoleamine 2, 3-dioxygenase at the transcriptional and post-transcriptional levels" [Int. J. Biochem. Cell Biol. 42 (2010) 1840-1846].

The international journal of biochemistry & cell biology·2026
Same journal

Whole-brain spatial metabolomics reveals metabolic gradient shifts in a murine glioma model following boron neutron capture therapy (130 characters).

The international journal of biochemistry & cell biology·2026
Same journal

LCN2 modulates Th17/Treg balance in vitro and is associated with an adaptive response to intestinal ischemia-reperfusion injury under Hmox1-deficient conditions.

The international journal of biochemistry & cell biology·2026
Same journal

Chloroquine modulates the redox-sensitive signalling via inhibiting the AMPK-ULK1 under LPS induced state in murine splenic macrophages.

The international journal of biochemistry & cell biology·2026
Same journal

The vicious cycle of hyperglycemia and oxidative stress: Novel mechanistic insights into a pathogenic alliance.

The international journal of biochemistry & cell biology·2026
Same journal

Fibroblast growth factors (FGF) in the pathogenesis and treatment of inflammatory bowel diseases (IBD): An overview.

The international journal of biochemistry & cell biology·2026
See all related articles

Related Experiment Video

Updated: Feb 9, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K

SRRF: Universal live-cell super-resolution microscopy.

Siân Culley1, Kalina L Tosheva2, Pedro Matos Pereira1

  • 1MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK; Department of Cell and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK; The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK.

The International Journal of Biochemistry & Cell Biology
|June 1, 2018
PubMed
Summary
This summary is machine-generated.

Super-Resolution Radial Fluctuations (SRRF) makes live-cell super-resolution microscopy accessible. This novel method works with standard microscopes, enabling detailed imaging of dynamic biological processes with minimal photobleaching.

Keywords:
FluorescenceImage processingLive-cell imagingSuper-resolution microscopy

More Related Videos

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.6K

Related Experiment Videos

Last Updated: Feb 9, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Test Samples for Optimizing STORM Super-Resolution Microscopy
16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

31.6K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Super-resolution microscopy offers nanoscale resolution with light microscopy benefits.
  • Current live-cell super-resolution techniques are often complex and hardware-intensive.
  • Bridging the gap between resolution and live-cell imaging accessibility is crucial.

Purpose of the Study:

  • To introduce a novel analytical approach, Super-Resolution Radial Fluctuations (SRRF).
  • To demonstrate the accessibility of live-cell super-resolution microscopy using SRRF.
  • To validate SRRF's applicability across different microscopy setups.

Main Methods:

  • Development and application of the Super-Resolution Radial Fluctuations (SRRF) analytical method.
  • Imaging of live samples expressing GFP.
  • Utilisation of commercial confocal, laser-based widefield, and LED-based widefield microscopes.

Main Results:

  • SRRF successfully achieved super-resolution imaging in live samples.
  • The method demonstrated applicability with standard confocal and widefield microscopes.
  • LED-based widefield microscopy with SRRF enabled long-term timelapse imaging with reduced photobleaching.

Conclusions:

  • SRRF significantly enhances the accessibility of live-cell super-resolution microscopy.
  • The technique integrates with existing, non-specialised microscopy hardware.
  • SRRF facilitates advanced live-cell imaging studies with improved ease and reduced phototoxicity.