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Related Experiment Video

Updated: Feb 9, 2026

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
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The human CSF pain proteome.

Payam Emami Khoonsari1, Elena Ossipova2, Johan Lengqvist2

  • 1Department of Medical Sciences, Clinical Chemistry, Uppsala University, Sweden; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.

Journal of Proteomics
|June 1, 2018
PubMed
Summary

Researchers identified ten novel proteins in cerebrospinal fluid (CSF) associated with chronic pain in fibromyalgia and rheumatoid arthritis patients. These proteins may serve as potential biomarkers for diagnosing and understanding chronic pain mechanisms.

Keywords:
Cerebrospinal fluidChronic painFibromyalgiaPainProteomeRheumatoid arthritis

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Area of Science:

  • Neuroscience
  • Proteomics
  • Biomarker Discovery

Background:

  • Chronic pain affects over 1.5 billion people globally, posing a significant health challenge.
  • Current diagnosis of chronic pain conditions like fibromyalgia (FM) lacks specific biomarkers, relying on clinical assessments.
  • Cerebrospinal fluid (CSF) is a potential source for biomarkers related to nervous system diseases, including chronic pain.

Purpose of the Study:

  • To investigate proteins in human CSF associated with known pain genes.
  • To explore quantitative differences in CSF proteomes between patients with fibromyalgia (FM), rheumatoid arthritis (RA), and non-pain controls.
  • To identify potential protein biomarkers for dysfunctional and inflammatory chronic pain.

Main Methods:

  • Semi-quantitative proteomics using mass spectrometry (MS) to analyze CSF samples.
  • Multivariate data analysis to identify quantitative differences in protein expression.
  • Comparison of CSF proteomes from FM patients, RA patients, and healthy controls.

Main Results:

  • Proteins detected in CSF were predominantly linked to synaptic transmission, inflammatory responses, neuropeptide signaling, and hormonal activity.
  • Identified ten proteins potentially associated with chronic pain in FM and RA patients.
  • These ten proteins include neural cell adhesion molecule L1, complement C4-A, lysozyme C, and others involved in inflammatory response and signal transduction.

Conclusions:

  • The identified proteins may aid in understanding the mechanisms of chronic pain in FM and RA.
  • These proteins represent potential novel biomarkers for diagnosing and managing chronic pain conditions.
  • Further research utilizing CSF and MS-based proteomics can advance chronic pain research and clinical practice.