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Related Experiment Videos

Distinct rotaviruses isolated from asymptomatic calves.

S Dea, M E Begin, D Archambault

    The Cornell Veterinarian
    |April 1, 1985
    PubMed
    Summary

    Four non-diarrheic calf rotaviruses were isolated and propagated. While antigenically related to Nebraska calf rotavirus, three isolates showed distinct thermal stability or antibody responses, and two had different plaque sizes.

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    Area of Science:

    • Veterinary Virology
    • Gastroenterology

    Background:

    • Rotaviruses are a significant cause of diarrhea in calves.
    • Understanding calf rotavirus strains is crucial for disease control.

    Purpose of the Study:

    • To isolate and characterize rotaviruses from non-diarrheic calves.
    • To compare these isolates with the Nebraska calf rotavirus reference strain.

    Main Methods:

    • Cell culture of intestinal contents.
    • Serial propagation in MDBK and BSC-1 cells.
    • Immunofluorescence, thermal stability assays, and plaque assays.

    Main Results:

    • Four rotavirus isolates were obtained from non-diarrheic calves.
    • Isolates were morphologically similar to pathogenic rotaviruses and antigenically related to Nebraska calf rotavirus.
    • Three isolates differed in thermal stability or response to neutralizing antibodies; two showed different plaque sizes.

    Conclusions:

    • Calf rotaviruses can be isolated from non-diarrheic animals.
    • Distinct antigenic and phenotypic variations exist among calf rotavirus strains.
    • Further characterization is needed to understand the full spectrum of calf rotavirus diversity.

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