Related Experiment Video

Updated: Feb 9, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K

Identification and functional characterization of circRNA-0008717 as an oncogene in osteosarcoma through sponging

Xiang Zhou1, Dimple Natino2, Zili Qin3

  • 1Department of Orthopaedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Oncotarget
|June 2, 2018
PubMed

Insights

Circular RNA (circRNA) acts as an oncogene in osteosarcoma, promoting tumor growth and invasion. Silencing circRNA-0008717 offers a potential therapeutic strategy for osteosarcoma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in human cancer development.
  • The specific role of circRNAs in osteosarcoma tumorigenesis remains largely unelucidated.

Purpose of the Study:

  • To investigate the expression and function of circRNAs in osteosarcoma.
  • To identify specific circRNAs involved in osteosarcoma carcinogenesis.

Main Methods:

  • Human circRNA microarray analysis to screen differentially expressed circRNAs.
  • Gain- and loss-of-function assays to assess circRNA-0008717's biological activity.
  • RNA immunoprecipitation (RIP) to identify interacting microRNAs and downstream targets.

Main Results:

  • circRNA-0008717 was significantly upregulated in osteosarcoma tissues and associated with poor patient survival.
  • Knockdown of circRNA-0008717 inhibited osteosarcoma cell proliferation, migration, and invasion, while promoting apoptosis.
  • circRNA-0008717 sponged miR-203, leading to increased Bmi-1 expression and subsequent promotion of osteosarcoma progression.

Conclusions:

  • circRNA-0008717 functions as an oncogene in osteosarcoma.
  • circRNA-0008717 is a potential prognostic biomarker for osteosarcoma.
  • Targeting circRNA-0008717 presents a novel therapeutic avenue for osteosarcoma treatment.
Keywords:
Bmi-1circRNA-0008717miR-203osteosarcoma

More Related Videos

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.1K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.7K

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.5K
Functional Groups02:45

Functional Groups

Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
89.1K
Functional Groups02:45

Functional Groups

24.5K
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.2K
Functionalism01:11

Functionalism

William James, John Dewey, and Charles Sanders Peirce were instrumental in founding functional psychology, which draws heavily from Darwin's theory of evolution by natural selection. This theory suggests that individual traits, including behaviors, are adapted to their environments through natural selection. At the heart of functionalism is the concept of adaptation, meaning that a trait enhances an individual's chances of survival and reproduction.
James envisioned psychology's...
2.7K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.5K