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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
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In vivo time-gated diffuse correlation spectroscopy at quasi-null source-detector separation.

M Pagliazzi, S Konugolu Venkata Sekar, L Di Sieno

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    We developed a new time-gated diffuse correlation spectroscopy method for measuring blood flow. This technique simplifies instrumentation and reduces probe size for enhanced in-vivo measurements.

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    Area of Science:

    • Biomedical Optics
    • Medical Instrumentation
    • Physiological Monitoring

    Background:

    • Diffuse correlation spectroscopy (DCS) is crucial for non-invasively assessing tissue blood flow.
    • Traditional DCS systems often require complex time-tagging electronics and can be limited by probe size and photon collection efficiency.

    Purpose of the Study:

    • To demonstrate a simplified time-domain diffuse correlation spectroscopy (TD-DCS) approach.
    • To enable quasi-null source-detector separation measurements for enhanced blood flow detection.
    • To reduce instrumentation complexity and probe dimensions for practical applications.

    Main Methods:

    • Utilized a fast time-gated single-photon avalanche diode (SPAD) detector.
    • Implemented TD-DCS at quasi-null source-detector separation.
    • Eliminated the need for conventional time-tagging electronics.

    Main Results:

    • Achieved increased photon collection efficiency.
    • Demonstrated simplified real-time instrumentation and reduced probe dimensions.
    • Successfully performed depth-discriminating, quasi-null distance blood flow measurements in a human subject.

    Conclusions:

    • The developed TD-DCS method offers a more practical and efficient approach for blood flow monitoring.
    • This technique paves the way for miniaturized optical sensors with improved spatial resolution.
    • Enhanced contrast of local blood flow changes can be achieved for advanced physiological monitoring.