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Related Experiment Videos

IgG subclass antibodies to herpes simplex virus.

R M Coleman, A J Nahmias, S C Williams

    The Journal of Infectious Diseases
    |May 1, 1985
    PubMed
    Summary

    This study evaluated monoclonal antibodies for detecting human IgG subclasses against herpes simplex virus (HSV). IgG1 antibodies were common in HSV infections, while IgG2, IgG3, and IgG4 appeared in recurrent cases, with IgG4 more frequent in men.

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    Area of Science:

    • Immunology
    • Virology
    • Infectious Diseases

    Background:

    • Human IgG antibodies are crucial for adaptive immunity against viral infections like herpes simplex virus (HSV).
    • Understanding IgG subclass responses (IgG1-IgG4) to HSV can provide insights into disease pathogenesis and immune status.
    • The availability of well-characterized reagents is essential for accurate immunological assays.

    Purpose of the Study:

    • To evaluate the utility of mouse monoclonal antibodies in detecting human IgG subclass antibodies against HSV antigens.
    • To investigate the prevalence and patterns of IgG subclass responses in patients with various forms of HSV infections.

    Main Methods:

    • Enzyme-linked immunosorbent assay (ELISA) was employed to detect human IgG subclass antibodies.
    • Several mouse monoclonal antibodies specific for human IgG1, IgG2, IgG3, and IgG4 were utilized.

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  • A total of 204 sera from 157 HSV-infected patients and controls were analyzed.

    • Main Results:

    • Human IgG1 antibodies were detected in nearly all HSV-infected individuals and were the initial responders in primary genital infections.
    • IgG2, IgG3, and IgG4 antibodies were found in acute-phase sera, predominantly in patients with recurrent genital herpes, but absent in primary infections.
    • A significantly higher frequency of IgG4 antibodies was observed in men compared to women with recurrent genital herpes.

    Conclusions:

    • The study highlights the need for well-characterized monoclonal antibodies for reliable detection of antibody responses to infectious agents.
    • Distinct patterns of IgG subclass antibody responses to HSV were observed in primary versus recurrent infections.
    • Sex-based differences in IgG4 antibody prevalence were noted in recurrent genital herpes, suggesting potential immunomodulatory factors.