Induction of human interferon gamma in nylon-column nonadherent human lymphocyte cells by heat-treated poly I:poly C

Human peripheral blood mononuclear leukocytes (PBML) were separated by Ficoll-hypaque gradient from human peripheral whole blood cells. The PBML were further separated by nylon-column. When nylon-column nonadherent cells (NNA cells) were induced by poly I:poly C which was prepared by heat-treatment, high levels of acid- and heat-labile interferon (IFN) were obtained. The IFN was not neutralized with human IFN-alpha or -beta anti-serum, but was completely neutralized with human IFN-gamma anti-serum, indicating that the IFN was human IFN-gamma.