Effect of complement component 5 polymorphisms on mastitis resistance in Egyptian buffalo and cattle
Nermin El-Halawany1, Abd-El-Monsif A Shawky1, Ahmed F M Al-Tohamy1
1Department of Cell Biology, National Research Centre, Dokki, 12622 Giza, Egypt.
Research in Veterinary Science
|June 2, 2018
Summary
Researchers identified genetic variations in the complement Component 5 (C5) gene in Egyptian buffalo and cattle. Specific C5 gene variants in buffalo are linked to mastitis resistance, offering insights into dairy animal health.
Area of Science:
- Veterinary Immunology
- Dairy Science
- Genetics
Background:
- Mastitis significantly impacts the global dairy industry, causing substantial economic losses.
- Complement Component 5 (C5) is crucial for phagocytosis and innate immunity in the bovine mammary gland, potentially influencing mastitis resistance.
- Understanding genetic factors associated with mastitis is vital for improving dairy animal health and productivity.
Purpose of the Study:
- To sequence and analyze the complete coding region of the C5 gene in Egyptian buffalo and cattle.
- To identify nucleotide variations (polymorphisms) within the C5 gene.
- To investigate the association between C5 gene variants and milk somatic cell score (SCS) as an indicator of mastitis.
Main Methods:
- Sequencing of buffalo and cattle C5 cDNA fragments, including the whole coding region and 3-UTR.
- Bioinformatic analysis to identify single nucleotide polymorphisms (SNPs).
- Statistical association analysis between identified SNPs and milk somatic cell score (SCS).
Main Results:
- Sequenced buffalo C5 cDNA (5336 bp) and cattle C5 cDNA (5303 bp) with 99% sequence identity.
- Identified nine novel SNPs in buffalo and seven SNPs in cattle C5 genes.
- Five SNPs in buffalo C5 were significantly associated with SCS, suggesting a role in mastitis resistance; no significant associations were found in cattle.
Conclusions:
- The study reports novel C5 gene variants in Egyptian buffalo and cattle.
- Specific C5 variants in buffalo are associated with mastitis resistance, indicating their potential as genetic markers.
- Further research is needed to explore the role of C5 variants in cattle mastitis resistance.
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