Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three restriction endonucleases from Anabaena flos-aquae.

P R Whitehead, N L Brown

    Journal of General Microbiology
    |April 1, 1985
    PubMed
    Summary

    Researchers purified three novel restriction enzymes, AflII and AflIII, from Anabaena flos-aquae. These enzymes offer new DNA analysis and molecular cloning applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Molecular characterization of the gor gene encoding glutathione reductase from Pseudomonas aeruginosa: determinants of substrate specificity among pyridine nucleotide-disulphide oxidoreductases.

    Molecular microbiology·2017
    Same author

    Sfpi1/PU.1 mutations in mouse radiation-induced acute myeloid leukaemias affect mRNA and protein abundance and associate with disrupted transcription.

    Leukemia research·2010
    Same author

    Tails of the unexpected: palatal medial edge epithelium is no more specialized than other embryonic epithelium.

    Orthodontics & craniofacial research·2007
    Same author

    A new approach to the remediation of heavy metal liquid wastes via off-gases produced by Klebsiella pneumoniae M426.

    Biotechnology and bioengineering·2006
    Same author

    A new approach for the recovery of precious metals from solution and from leachates derived from electronic scrap.

    Biotechnology and bioengineering·2006
    Same author

    Mercury tolerance of thermophilic Bacillus sp. and Ureibacillus sp.

    Biotechnology letters·2005

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Microbiology

    Background:

    • Cyanobacteria are prokaryotic microorganisms with diverse metabolic capabilities.
    • Restriction endonucleases are enzymes that cleave DNA at specific recognition sites.
    • Understanding enzyme specificities is crucial for molecular biology applications.

    Purpose of the Study:

    • To identify and characterize novel restriction endonucleases from the cyanobacterium Anabaena flos-aquae.
    • To determine the recognition and cleavage specificities of these enzymes.
    • To assess the potential utility of these enzymes in molecular cloning and DNA analysis.

    Main Methods:

    • Partial purification of endonucleases from Anabaena flos-aquae CCAP 1403/13f.
    • Determination of enzyme recognition sequences through DNA digestion assays.
    • Analysis of cleavage patterns to define enzyme specificities.

    Main Results:

    • Three site-specific endonucleases, designated AflI, AflII, and AflIII, were partially purified.
    • The specific recognition and cleavage sequences for AflI, AflII, and AflIII were determined.
    • AflII and AflIII represent novel specificities not previously described.

    Conclusions:

    • The newly identified restriction enzymes AflII and AflIII possess unique specificities.
    • These enzymes hold potential for applications in molecular cloning techniques.
    • Further analysis of DNA can be facilitated by these novel restriction endonucleases.

    Related Experiment Videos