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Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells
Published on: June 14, 2017
In vitro-microenvironment directs preconditioning of human chorion derived MSC promoting differentiation of OPC-like
Ramesh Periasamy1, Daniel V Surbek1, Andreina Schoeberlein1
1Department of Obstetrics and Gynecology, University Hospital Bern, University of Bern, Switzerland.
Abstract:
The loss of oligodendrocyte progenitor cells (OPC) is a hallmark of perinatal brain injury. Our aim was to develop an in vitro culture condition for human chorion-derived mesenchymal stem cells (MSC) that enhances their stem cell properties and their capability to differentiate towards OPC-like cells. MSC were grown either in serum replacement medium (SRM) or serum-containing medium (SM) and tested for their morphology, proliferation, secretome, migration, protein expression and differentiation into OPC-like cells. MSC cultured in SRM condition have distinct morphology/protein expression profile, increased cell proliferation/migration and capacity to differentiate into OPC-like cells.
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