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Updated: Feb 9, 2026
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IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol PEG Precipitation
Published on: May 1, 2011
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Isolation of IgY from Chicken Eggs
Cold Spring Harbor Protocols
|June 3, 2018
Summary
Chicken egg yolk antibody IgY isolation requires specific methods, as it doesn't bind Protein A or G. This protocol details two precipitation techniques using sodium sulfate or Polyethylene Glycol 6000 for efficient IgY purification.
Area of Science:
- Immunology
- Biochemistry
Background:
- Immunoglobulin Y (IgY) is the predominant antibody class in birds and reptiles.
- Unlike mammalian Immunoglobulin G (IgG), IgY does not bind to Protein A or Protein G, necessitating alternative purification strategies.
Purpose of the Study:
- To describe a reliable protocol for the isolation of chicken IgY from egg yolks.
- To present two distinct differential precipitation methods for IgY purification.
Main Methods:
- Separation of egg yolk from egg white.
- Differential precipitation of yolk proteins using sodium sulfate.
- Alternative differential precipitation using Polyethylene Glycol 6000.
Main Results:
- Both sodium sulfate and Polyethylene Glycol 6000 effectively precipitate contaminating proteins, allowing for IgY isolation.
- The methods ensure IgY is isolated within a 2-3 week timeframe to prevent degradation.
Conclusions:
- The described methods provide a practical approach for obtaining purified chicken IgY.
- These protocols are essential for researchers utilizing IgY in various immunological applications.
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