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Updated: Feb 9, 2026

In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging
Ajit Dhadve1,2, Bhushan Thakur1,3, Pritha Ray4,5
1Imaging Cell Signaling & Therapeutics Lab, Tata Memorial Centre (TMC), Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Navi Mumbai, Maharashtra, India.
Abstract:
Dual modality reporter genes are powerful means of tracking cellular processes in cell culture systems and whole animals. In this chapter, we describe the methods for construction of a plasmid reporter gene vector expressing a fluorescent and a bioluminescent gene and its validation by in vitro assays in mammalian cells as well as by noninvasive imaging methods in small animal models.
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