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Updated: Feb 9, 2026

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT
Published on: June 1, 2011
Visualization of Immune Cell Reconstitution by Bioluminescent Imaging
1Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA. purnima.dubey@osumc.edu.
Abstract:
Reporter gene-based molecular imaging is a powerful means to detect the movement and function of diverse cell populations in vivo. Reconstitution of an immune system marked with molecular imaging reporter genes permits tracking of primary immune responses to pathogens and cancer in experimental systems. This chapter describes the methods to isolate bone marrow stem/progenitors, transduce them with imaging reporter genes, and track the reconstitution of the peripheral immune system by bioluminescent imaging.
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