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Updated: Feb 9, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Published on: October 27, 2011
In Silico Typing of Classical and Non-classical HLA Alleles from Standard RNA-Seq Reads
Sebastian Boegel1, Thomas Bukur2, John C Castle2,3
1TRON gGmbH - Translational Oncology at Johannes Gutenberg-University Medical Center gGmbH, Mainz, Germany. seb.boegel@gmail.com.
Abstract:
Next-Generation Sequencing (NGS) enables the rapid generation of billions of short nucleic acid sequence fragments (i.e., "sequencing reads"). Especially, the adoption of gene expression profiling using whole transcriptome sequencing (i.e., "RNA-Seq") has been rapid. Here, we describe an in silico method, seq2HLA, that takes standard RNA-Seq reads as input and determines a sample's (classical and non-classical) HLA class I and class II types as well as HLA expression. We demonstrate the application of seq2HLA using publicly available RNA-Seq data from the Burkitt's lymphoma cell line DAUDI and the choriocarcinoma cell line JEG-3.
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