Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
Distance Corrections01:15

Distance Corrections

299
To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
299
Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

11.0K
In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
11.0K
Fundamental Attribution Error01:14

Fundamental Attribution Error

13.8K
According to some social psychologists, people tend to overemphasize internal factors as explanations—or attributions—for the behavior of other people. They tend to assume that the behavior of another person is a trait of that person, and to underestimate the power of the situation on the behavior of others. They tend to fail to recognize when the behavior of another is due to situational variables, and thus to the person’s state. This erroneous assumption is...
13.8K
Power Factor Correction01:20

Power Factor Correction

548
The power transmission to a factory involves the transfer of apparent power, a combination of active and reactive power. The power factor measures how effectively electrical power is converted into useful work output. The ratio of the real power (KW) that does the work to the apparent power (KVA) supplied to the circuit.
548
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.2K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of O-antigen due to <i>wbbL</i> mutations is common and associated with increased mortality in <i>Escherichia coli</i> bloodstream infections.

Science advances·2026
Same author

Diagnostic utility of clinical genome reanalysis in rare pediatric disorders using long-read sequencing.

HGG advances·2026
Same author

Bringing the genetically minimal cell to life on a computer in 4D.

Cell·2026
Same author

Selection-free whole genome transplantation revives dead microbes.

bioRxiv : the preprint server for biology·2026
Same author

Bringing the genetically minimal cell to life on a computer in 4D.

Cell·2026
Same author

IFN signaling is associated with radiotherapy response in malignant peripheral nerve sheath tumors.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Feb 9, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.9K

DNA assembly with error correction on a droplet digital microfluidics platform.

Yuliya Khilko1,2, Philip D Weyman3, John I Glass3

  • 1Stanford Genome Technology Center, Stanford University, 3165 Porter Drive, Palo Alto, CA, 94304, USA.

BMC Biotechnology
|June 3, 2018
PubMed
Summary

This study developed an automated digital microfluidic (DMF) platform for DNA synthesis, reducing costs and labor. The DMF platform successfully assembled DNA oligonucleotides and improved sequence fidelity through error correction.

Keywords:
DNADigital microfluidicsError correctionGibson assembly

More Related Videos

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K
A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education
05:46

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education

Published on: April 26, 2021

5.3K

Related Experiment Videos

Last Updated: Feb 9, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.9K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.6K
A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education
05:46

A Versatile Kit Based on Digital Microfluidics Droplet Actuation for Science Education

Published on: April 26, 2021

5.3K

Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Custom DNA synthesis is crucial for synthetic biology but is currently expensive and labor-intensive.
  • Microfluidic technologies offer automation and reduced sample volumes, potentially lowering DNA synthesis costs.
  • This study aimed to develop a digital microfluidic (DMF) gene assembly protocol.

Purpose of the Study:

  • To adapt and optimize bench-scale oligonucleotide assembly and enzymatic error correction methods for the Mondrian™ DMF platform.
  • To demonstrate the feasibility of automated, microfluidic-based DNA synthesis.

Main Methods:

  • Adapted Gibson assembly, polymerase chain reaction (PCR), and enzymatic error correction for DMF.
  • Optimized reactions for small volumes (0.6–1.2 μL) on the Mondrian™ platform.
  • Assembled 12 oligonucleotides into a 339-bp DNA sequence encoding part of the human influenza virus hemagglutinin (HA) gene.

Main Results:

  • Successfully assembled oligonucleotides into the target DNA sequence on the DMF platform.
  • Achieved an initial error frequency of approximately 4 errors/kb, reduced to 1.8 errors/kb after one round of error correction.
  • Optimized PCR required increased MgCl2, Phusion polymerase, and PEG 8000 compared to benchtop methods.

Conclusions:

  • Demonstrated complete automation of DNA assembly and error correction on a DMF platform.
  • Enzymatic reactions in droplets required surfactants, molecular crowding agents, and excess enzyme for successful on-chip implementation.
  • Enzymatic error correction improved sequence fidelity twofold, indicating potential for further optimization.