Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

3.1K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
3.1K
Chromatography: Introduction01:10

Chromatography: Introduction

7.2K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
7.2K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

2.2K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
2.2K
Types Of Column Chromatography01:29

Types Of Column Chromatography

13.9K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
13.9K
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

9.0K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
9.0K
Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

1.3K
In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial for Special Issue "Recombinant Proteins for Molecular Biology Research: Technologies and Applications".

Current issues in molecular biology·2026
Same author

Parameter optimization for circular dichroism spectroscopy of proteins: A practical approach for rapid acquisition of high-quality spectra.

Analytical biochemistry·2026
Same author

A robust and sensitive method for detecting subtle structural differences in bovine serum albumin.

BioTechniques·2026
Same author

Effects of Arginine on Hierarchical Protein Aggregation.

The protein journal·2026
Same author

Differences and Similarities in Protein and Nucleic Acid Structures and Their Biological Interactions.

Current issues in molecular biology·2026
Same author

Non-ionic Detergent-Assisted Refolding of Protein from Protein-SDS Complex.

The protein journal·2025

Related Experiment Video

Updated: Feb 9, 2026

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K

Excluded Cosolvent in Chromatography.

Tsutomu Arakawa1, Pete Gagnon2

  • 1Alliance Protein Laboratories, a Division of KBI Biopharma, 6042 Cornerstone Court West, San Diego, California 92121.

Journal of Pharmaceutical Sciences
|June 4, 2018
PubMed
Summary
This summary is machine-generated.

Cosolvent exclusion, a concept from the 1970s-1990s, explains how cosolvents affect macromolecule stability and solubility. This principle is now enhancing protein separation in various chromatography techniques.

Keywords:
aggregate removalchromatographycosolventexclusionformulationpreferential interaction

More Related Videos

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

38.0K
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.6K

Related Experiment Videos

Last Updated: Feb 9, 2026

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K
Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

38.0K
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.6K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Cosolvent exclusion theory, developed by Timasheff's lab (1970-1990), explains macromolecule stability and solubility.
  • This concept has significantly impacted formulation and protein folding studies.
  • It is increasingly applied in chromatography to improve separation of biomolecules.

Purpose of the Study:

  • To review the principles of preferential cosolvent exclusion.
  • To summarize its applications in various column chromatography methods.
  • To highlight the emergence of steric exclusion chromatography.

Main Methods:

  • Review of existing literature on cosolvent exclusion.
  • Analysis of cosolvent effects in different chromatography modes (ion exchange, hydroxyapatite, affinity, hydrophobic interaction).
  • Discussion of steric exclusion chromatography utilizing hydroxylated surfaces and polyethylene glycol (PEG).

Main Results:

  • Excluded cosolvents, including salts, amino acids, and PEG, enhance binding and resolution of macromolecules.
  • Steric exclusion chromatography uses PEG to stabilize macromolecule-solid phase interactions.
  • Elution in steric exclusion chromatography is controlled by decreasing PEG concentration.

Conclusions:

  • Preferential cosolvent exclusion is a versatile concept with broad applications in chromatography.
  • Steric exclusion chromatography offers a novel approach for macromolecule separation.
  • The use of cosolvents like PEG significantly influences chromatographic performance and separation efficiency.