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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
BODIPY-based turn-on fluorescent probes for cysteine and homocysteine
Jinhua Gao1, Yuanfang Tao1, Nannan Wang1
1Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng 475004, PR China.
Abstract:
Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are interconnected and play essential roles in many biological processes. It is significant importance to detect these thiols for investigating their functions in cells and disease diagnosis. In this work, we have designed and synthesized two novel BODIPY-based turn-on fluorescent probes (BDP-Ph and BDP-R-Ph) carrying 4-methoxythiophenol moiety at meso position as good leaving group for highly selective detection of Cys and Hcy. Furthermore, the probes have been successfully applied to detect intracellular Cys and Hcy by fluorescent imaging in living cells.
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