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Published on: March 6, 2013
pH-Dependent stability of azithromycin in aqueous solution and structure identification of two new degradation
Maria Grazia Saita1, Danilo Aleo1, Barbara Melilli1
1MEDIVIS - Corso Italia, 171-95127, Catania, Italy.
Abstract:
The degradation profile of azithromycin in buffered solutions was investigated using HPLC and found to be pH dependent in the range of 6.0-7.2. Desosaminylazitromycin, derived from hydrolytic loss of cladinose of the parent molecule, was the major degradation product at pH 6.0 but its amount progressively decreased moving toward pH 7.2. Two additional unreported degradation products were also observed and their structures were fully elucidated by MS- and NMR-spectroscopy to be associated with opening of the macrocyclic lactone ring.
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