[Simultaneous quantification of 7 coumarins in common cnidium fruit by GC-MS]

A GC-MS-SIM method was developed for the simultaneous determination of the 7 coumarins in common cnidium fruit. The 7 bioactive constituents were separated on DB-1 capillary column（30 m × 0.25 mm, 0.25 μm） using temperature programming. The interface temperature was set at 280 ℃; Ion source temperature: 250 ℃; Quadrupole temperature: 150 ℃; EI mode: 70 e V; The mass spectrometer detector was in SIM mode; Scan range: 50-350 amu. All the 7 marker substances showed good linearity（r(2) > 0.998 6） in the test ranges. The LODs and LOQs for the compounds ranged from 1.06 to 10.11 ng·mL(-1) and from 3.21 to 29.88 ng·m L(-1), respectively. The overall intra-day（n = 6） and inter-day（n = 3） RSDs were 0.7%-2.5% and 1.2%-3.3%, respectively. The overall recoveries were between 92.38% and 100.50% for all compounds. This method was simple, rapid, sensitive, with good specificity, and it can provide a reference for the quality control of common cnidium fruit.