Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

4.4K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.4K
Molecules and Compounds02:38

Molecules and Compounds

69.0K
Atoms and Molecules
69.0K
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

9.7K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
9.7K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.6K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.6K
Positive Regulator Molecules01:45

Positive Regulator Molecules

136.5K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective Efficacy of Met-Ser-Arg Against Irradiation-Related Testicular Injury†.

Biology of reproduction·2026
Same author

Sustained and Uniform Self-transport of Condensate Droplets via a Bionic Dual-Gradient Wedged Groove.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Fbxo2 suppresses prostate cancer progression by regulating YTHDF2 ubiquitination and degradation.

Cell death & disease·2025
Same author

Laparoscopic-based renal sympathetic denervation for the management of refractory hypertension in patients with end-stage renal disease: A case series of three patients.

Clinical nephrology·2025
Same author

A chromosomal-level genome assembly of Thanatophilus sinuatus Fabricius (Coleoptera: Staphylinidae).

Scientific data·2025
Same author

Bibliometric analysis of magnetic resonance imaging-ultrasound fusion in prostate biopsy: trends and collaborations (2000-2024).

Lasers in medical science·2025

Related Experiment Video

Updated: Feb 9, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.8K

Intranucleus Single-Molecule Imaging in Living Cells.

Shipeng Shao1, Boxin Xue1, Yujie Sun1

  • 1State Key Laboratory of Membrane Biology, BIOPIC, School of Life Sciences, Peking University, Beijing, China.

Biophysical Journal
|June 5, 2018
PubMed
Summary

Intranuclear single-molecule imaging allows direct observation of cellular processes like transcription and DNA repair. Recent advancements in labeling and imaging methods are enabling new insights into live cell nuclei.

More Related Videos

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

18.3K

Related Experiment Videos

Last Updated: Feb 9, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.8K
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

18.3K

Area of Science:

  • Cellular Biology
  • Molecular Imaging
  • Biophysics

Background:

  • Many critical cellular processes are stochastic and best studied at the single-molecule level.
  • Ensemble measurements obscure crucial details of nuclear functions like transcription, replication, and DNA repair.
  • Studying these nuclear processes requires advanced intranuclear imaging techniques.

Purpose of the Study:

  • To review recent methodological developments in single-molecule imaging for live cell nuclei.
  • To highlight emerging applications of these techniques in nuclear research.
  • To discuss current challenges and future perspectives in the field.

Main Methods:

  • Focus on advancements in labeling strategies for single-molecule detection within the nucleus.
  • Describe novel imaging techniques enabling visualization of molecular events in real-time.
  • Discuss methodologies for analyzing stochastic processes at the single-molecule level in vivo.

Main Results:

  • Significant progress has been made in developing tools for intranuclear single-molecule imaging over the past decade.
  • Emerging applications demonstrate the power of these methods to reveal nuclear dynamics.
  • Despite progress, challenges in labeling and imaging persist, limiting the scope of current studies.

Conclusions:

  • Single-molecule imaging offers unprecedented views into nuclear processes, overcoming limitations of ensemble studies.
  • Continued methodological development is crucial for expanding the application of these techniques.
  • Future research will likely focus on refining imaging tools and exploring new biological questions in the live nucleus.