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Post-Exposure Protection in Mice against Sudan Virus by a Two Antibody Cocktail
Jeffrey W Froude1, Andrew S Herbert2, Thibaut Pelat3
1US Army Medical Research Institute for Infectious Disease (USAMRIID), Fort Detrick, MD 21702, USA. Jeffrey.w.froude2.mil@mail.mil.
Viruses
|June 5, 2018
Summary
Researchers developed cross-reactive antibodies effective against Sudan virus (SUDV) and Ebola virus (EBOV). These novel antibodies, produced cell-free, demonstrated protection against SUDV in mice, offering potential therapeutic avenues.
Area of Science:
- Virology
- Immunology
- Biotechnology
Background:
- Sudan virus (SUDV) and Ebola virus (EBOV) are dangerous Ebolavirus species responsible for numerous outbreaks.
- Effective treatments and vaccines against these viruses are urgently needed.
Purpose of the Study:
- To generate and characterize cross-reactive antibodies targeting both SUDV and EBOV.
- To assess the therapeutic potential of these antibodies against SUDV infection.
Main Methods:
- Immunization of cynomolgus macaques with SUDV glycoprotein.
- Construction and screening of antibody fragment phage display libraries.
- Production of recombinant antibodies in scFv-Fc format using a cell-free system.
- Evaluation of antibody efficacy in a SUDV mouse model.
Main Results:
- Identified 58 unique antibody clones with cross-reactivity to SUDV and EBOV.
- Demonstrated SUDV neutralization by selected antibody clones.
- Achieved complete protection against SUDV challenge in mice using a combination of two antibodies (X10B1/X10H2).
Conclusions:
- Developed novel, cross-reactive antibodies against SUDV and EBOV.
- Cell-free production of therapeutic antibodies is feasible.
- These antibodies show promise as potential therapeutics for Sudan virus infections.
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