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Carcinogen-induced insertion mutations in E. coli.

M Takeshita, H Van der Keyl, A P Grollman

    Progress in Clinical and Biological Research
    |January 1, 1985
    PubMed
    Summary

    The carcinogen acetylaminofluorene (AAF) increases the rate of DNA recombination in E. coli. This study observed higher frequencies of IS5 element transposition in AAF-modified DNA, suggesting carcinogen adducts promote genetic instability.

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    Biochemistry·2000

    Area of Science:

    • Molecular Biology
    • Genetics
    • Carcinogenesis

    Background:

    • Acetylaminofluorene (AAF) is a known carcinogen.
    • DNA adducts formed by carcinogens can influence genetic processes.
    • Transposable elements like IS5 play roles in genome dynamics.

    Purpose of the Study:

    • To investigate the mutagenic effects of AAF on bacterial DNA.
    • To determine if AAF-modified DNA influences the transposition of IS5 elements.
    • To assess the impact of DNA-carcinogen adducts on recombinational events.

    Main Methods:

    • Competent E. coli cells (wild type and recA strains) were transformed with plasmid DNA.
    • Plasmid DNA was regionally modified with the carcinogen AAF.
    • Analysis of plasmids for IS5 transposition and other mutations (insertions, deletions).

    Main Results:

    • IS5 transposition was detected in six plasmids from AAF-modified DNA.
    • A single GC addition mutation was found in one plasmid.
    • No deletions were observed in the analyzed plasmids.
    • The frequency of IS5 insertion (2.5 X 10(-3)) was higher than naturally occurring rates.

    Conclusions:

    • The presence of DNA-carcinogen adducts, specifically AAF, enhances the frequency of IS5 transposition in E. coli.
    • Carcinogen-induced DNA modifications can increase the rate of recombinational events.
    • These findings highlight a mechanism by which carcinogens may contribute to genetic instability and mutagenesis.

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