Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Seedless Vascular Plants03:24

Seedless Vascular Plants

67.3K
Seedless Vascular Plants Were the First Tall Plants on Earth
67.3K
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

16.7K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
16.7K
Vascular Spasm01:16

Vascular Spasm

3.7K
The vascular phase, also known as vasospasm, is the initial stage of hemostasis, crucial for preventing excessive bleeding when a blood vessel is injured. After a vessel is cut, nerves in the damaged area trigger pain and other sensory impulses. Simultaneously, the smooth muscles in the vessel wall contract, resulting in a vascular spasm. This contraction reduces the vessel's diameter at the injury site, slowing or stopping blood loss through the vessel wall. Vascular spasms typically last...
3.7K
Overview of the Vascular System01:20

Overview of the Vascular System

3.6K
The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
3.6K
Vascular Resistance01:20

Vascular Resistance

11.1K
Vascular resistance is a critical concept in understanding blood flow dynamics in the circulatory system. It refers to the resistance that blood encounters as it flows through the blood vessels. This resistance is a key factor in determining blood pressure and cardiac workload.
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...
11.1K
Non-vascular Seedless Plants02:26

Non-vascular Seedless Plants

71.8K
The diverse plant life on Earth—consisting of nearly 400,000 species—can be divided into three broad categories based on biological characteristics: nonvascular, seedless vascular, and seed plants.
71.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homologous cell type markers highlight subdivisions of the domestic chick hippocampal formation.

Scientific reports·2026
Same author

Microstructured chitosan mesh scaffold for efficient production of cell-cultured meat.

Scientific reports·2026
Same author

An innovative treatment for lung cancer using gene-engineered human-induced pluripotent stem cell-derived natural killer cells.

Cancer immunology, immunotherapy : CII·2026
Same author

A Human Kidney Tubuloid Model of Repeated Cisplatin-Induced Cellular Senescence and Fibrosis for Drug Screening.

Advanced healthcare materials·2025
Same author

The Auxiliary-Field Quantum Monte Carlo Method with Seniority-Zero Trial Wave Function.

Journal of chemical theory and computation·2025
Same author

Masao Washizu (1953-2025).

Nature biomedical engineering·2025

Related Experiment Video

Updated: Feb 9, 2026

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

4.6K

Engineering of vascularized 3D cell constructs to model cellular interactions through a vascular network.

Emi Sano1, Chihiro Mori1, Yuji Nashimoto1

  • 1Department of Micro Engineering, Kyoto University, Kyoto 615-8540, Japan.

Biomicrofluidics
|June 5, 2018
PubMed
Summary

This study presents a microfluidic method to create 3D vascularized spheroids for improved in vitro models. This vascular network enables nutrient transport and allows for the study of cancer cell migration in a bone-like microenvironment.

More Related Videos

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.5K
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

8.8K

Related Experiment Videos

Last Updated: Feb 9, 2026

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

4.6K
Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
09:23

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

21.5K
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

8.8K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Current in vitro 3D culture models lack functional vascular systems, limiting cellular viability and hindering realistic physiological studies.
  • Essential components like oxygen, nutrients, and cells require efficient transport, which is absent in existing models.

Purpose of the Study:

  • To develop a microfluidic method for generating perfusable 3D vascular networks within multicellular spheroids.
  • To create a functional vascular system for nutrient delivery and to model in vivo-like cellular interactions and drug delivery.

Main Methods:

  • A microfluidic device was used to embed multicellular spheroids (endothelial cells, fibroblasts, MSCs) within a hydrogel.
  • Endothelial cells were seeded to promote angiogenesis, forming vascular networks that connected to microchannels for perfusion.
  • A bone-like microenvironment was recapitulated using osteo-differentiated MSCs to study breast cancer cell migration.

Main Results:

  • A perfusable 3D vascular network was successfully formed within multicellular spheroids, enabling nutrient and cell transport.
  • Breast cancer cell migration towards spheroids was significantly higher in the bone-like microenvironment compared to undifferentiated MSCs.
  • The system demonstrated effective modeling of in vivo-like cellular migration and potential for drug delivery studies.

Conclusions:

  • The developed 3D vascularized spheroids-on-a-chip system offers a powerful tool for in vitro modeling of complex biological processes.
  • This technology facilitates the study of cellular interactions, drug delivery, and disease progression in more physiologically relevant environments.
  • The platform holds significant potential for advancing cancer research and personalized medicine.