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Updated: Feb 9, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
Published on: April 17, 2021
Microfluidic flow-based platforms for induction and analysis of dynamic shear-mediated platelet activation-Initial
Annalisa Dimasi1, Yana Roka-Moiia2, Filippo Consolo
1Department of Electronics, Information and Bioengineering, Politecnico di Milano, Via Golgi 39, 20133 Milano, Italy.
Abstract:
A microfluidic flow-based platform (μFP), able to stimulate platelets via exposure of shear stress patterns pertinent to cardiovascular devices and prostheses, was compared to the Hemodynamic Shearing Device (HSD)-a state-of-the-art bench-top system for exposure of platelets to defined levels and patterns of shear. Platelets were exposed to time-varying shear stress patterns in the two systems; in detail, platelets were recirculated in the μFP or stimulated in the HSD to replicate comparable exposure time. Shear-mediated platelet activation was evaluated via (i) the platelet activity state assay, allowing the measurement of platelet-mediated thrombin generation and associated prothrombotic tendencies, (ii) scanning electron microscopy to evaluate morphological changes of sheared platelets, and (iii) flow cytometry for the determination of platelet phosphatidylserine exposure as a marker of shear activation. The results revealed good matching and comparability between the two systems, with similar trends of platelet activation, formation of microaggregates, and analogous trends of activation marker exposure for both the HSD and microfluidic-stimulated samples. These findings support future translation of the microfluidic platform as a Point-of-Care facsimile system for the diagnosis of thrombotic risk in patients implanted with cardiovascular devices.
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