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In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
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On-Chip Endothelial Inflammatory Phenotyping
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Matrix-Embedded Endothelial Cells Attain a Progenitor-Like Phenotype.

Eytan Abraham1, Or Gadish2, Joseph W Franses2

  • 1Institute for Medical Engineering and Science, Massachusetts Institute of Technology, E25-438, Cambridge, MA 02139, USA. Department of Medicine, Brigham and Women's Hospital, Cardiovascular Division, Harvard Medical School, Boston, MA 02115, USA.

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|June 5, 2018
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Matrix-embedded endothelial cells (MEECs) mimic endothelial progenitor cells (EPCs), showing therapeutic potential for tissue repair. 3D culture is crucial for maintaining EPC phenotype in vitro.

Keywords:
3Dendothelial cellsendothelial progenitor cellsmatrix embeddingscaffolds

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biomaterials Science

Background:

  • Endothelial cells (ECs) cultured in 3D collagen scaffolds (matrix-embedded ECs, MEECs) show promise for tissue repair.
  • MEECs are hypothesized to adopt a unique phenotype distinct from traditional 2D cultures, resembling early progenitor cells.

Purpose of the Study:

  • To compare MEECs with 2D-cultured ECs and endothelial progenitor cells (EPCs).
  • To investigate the phenotypic and genetic similarities between MEECs and EPCs.
  • To determine the impact of 3D matrices on EPC phenotype maintenance.

Main Methods:

  • Comparative analysis of secretome, phenotype, and genetic profiles.
  • Assessment of CD34 expression as a progenitor cell marker.
  • Evaluation of endothelial progenitor cell (EPC) behavior in 2D versus 3D cultures.

Main Results:

  • MEECs exhibit significant alterations from 2D-ECs, adopting an EPC-like phenotype.
  • Elevated CD34 expression and similar gene/protein profiles confirm the EPC-like nature of MEECs.
  • EPCs lose their phenotype in 2D culture but regain it in 3D matrices.

Conclusions:

  • 3D matrices are essential for recapitulating the in vivo phenotype of EPCs.
  • MEECs offer a promising model for studying progenitor cell behavior.
  • Understanding EC-matrix interactions is key to optimizing MEEC-based therapies for clinical application.