Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Deaggregation of human platelets aggregated by thrombin.

R L Kinlough-Rathbone, D W Perry, M A Packham

    Thrombosis and Haemostasis
    |February 18, 1985
    PubMed
    Summary

    Human platelets undergoing release reactions can be deaggregated by specific combined treatments. These treatments target fibrinogen receptors, heparin-sensitive protein binding, and proteolysis-resistant linkages for effective platelet separation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characteristics, Origins, and Biosignature Preservation Potential of Carbonate-Bearing Rocks Within and Outside of Jezero Crater.

    Journal of geophysical research. Planets·2021
    Same author

    Earth-like Habitable Environments in the Subsurface of Mars.

    Astrobiology·2021
    Same author

    Hearing in the Mind's Ear: A PET Investigation of Musical Imagery and Perception.

    Journal of cognitive neuroscience·2013
    Same author

    Changes in Phosphoinositides in Rabbit Platelets during Clot Formation. Comparison of Platelets Stimulated by ADP or by Thrombin in the Presence of Polymerising Fibrin.

    Platelets·2010
    Same author

    Ontario's Mustard Report: Its Implications For Family Physicians.

    Canadian family physician Medecin de famille canadien·2010
    Same author

    Closing remarks / conclusion.

    Canadian Medical Association journal·2010

    Area of Science:

    • Hematology
    • Biochemistry
    • Cell Biology

    Background:

    • Human platelets aggregate after undergoing a release reaction, making deaggregation difficult.
    • Thrombin-induced release reaction in platelets leads to aggregation that resists simple reversal.

    Purpose of the Study:

    • To investigate conditions for deaggregating human platelets after a thrombin-induced release reaction.
    • To identify the mechanisms responsible for platelet linkage following extensive release.

    Main Methods:

    • Washed human platelets were treated with thrombin to induce a release reaction.
    • Deaggregation was attempted using combinations of agents targeting fibrinogen receptors (CP/CPK, apyrase), cAMP levels (PGE1), protein digestion (chymotrypsin), and released proteins (heparin).
    • Thrombin activity was neutralized with hirudin in some experiments.

    Related Experiment Videos

    Main Results:

    • Individual treatments did not cause deaggregation.
    • Combinations of CP/CPK (or apyrase) with chymotrypsin, or PGE1 with chymotrypsin, deaggregated platelets when thrombin was neutralized.
    • For higher thrombin concentrations, deaggregation required pre-treatment with heparin before thrombin exposure, in addition to other agents.

    Conclusions:

    • Platelet linkage after extensive release involves at least three distinct mechanisms.
    • These mechanisms include fibrinogen receptor involvement, a heparin-sensitive protein-binding reaction, and a proteolysis-sensitive linkage.
    • Combined inhibition of these pathways is necessary for effective platelet deaggregation.